A simple & Rapid Method For Detecting Bacterial Myrosinase Corresponding Protein Band

Myrosinases have significant scientific and medical implications. Unfortunately, detection purification of myrosinase from microbes requires the use highly cost substrates (glucosinolates) such as sinigrin expensive instruments Fast Protein Liquid Chromatography or ion exchange chromatography. In this work, we used only 20 mL bacterial culture supplemented with (10 mM) to obtain partially purified myrosinase. The crude protein extract was loaded onto native polyacrylamide gel putative band identified eluted. This step successfully minimised numbers bands extracts be further analysed. current method describes a simple, rapid effective protocol for isolation active myrosinases. Furthermore, our can step.